Background: Negative Pressure Wound Therapy (NPWT) is commonly utilized in damage control surgery for military casualties. However, its application during aeromedical evacuation has been linked to severe complications, including in-flight evisceration, largely due to improper usage by non-surgeon personnel who are unfamiliar with the technology. Current training resources, such as Clinical Practice Guidelines (CPGs), provide limited hands-on instruction, leading to critical knowledge gaps in troubleshooting NPWT failures. This study evaluates whether a targeted video curriculum (VC) improves NPWT competency compared to traditional CPG training. Methods: A multidisciplinary team developed a five-part video curriculum focused on NPWT functions and failure management. A total of 115 NPWT-naïve, non-surgical military medical personnel were randomized into two groups: VC and CPG. Participants individually identified NPWT components and subsequently worked in teams to troubleshoot leak, obstruction, and hemorrhage scenarios using an in vivo porcine model. Performance was assessed based on successful completion of critical action steps. Results: VC participants demonstrated superior competency in NPWT device identification (p<0.001), correct canister attachment (p<0.001), and leak troubleshooting (p=0.011). Teams trained with VC completed more critical steps in obstruction (p=0.001) and leak scenarios (p=0.011), addressing key failure points implicated in in-flight evisceration events. Post-study surveys indicated VC participants found the training more useful (p=0.008) and were more likely to recommend it to colleagues (p=0.02). Conclusions: Video-based training significantly improves NPWT competency among non-surgeon personnel, addressing key deficiencies associated with improper wound vac application during aeromedical transport. Implementation of video-based training across military medical units may reduce the incidence of NPWT-related complications, including in-flight evisceration. Disclaimer The views expressed in this research are those of the authors and do not necessarily reflect the official policy or position of the Department of Defense, its components, or the U.S. Government. The mention of specific products or technologies does not constitute endorsement by the DoD. The voluntary, fully informed consent of the subjects used in this research was obtained as required by 32 CFR 219 and DODI 3216.02_AFI 40-402. The experiments reported herein were conducted according to the principles set forth in the National Institute of Health Publication No. 80-23, Guide for the Care and Use of Laboratory Animals and the Animal Welfare Act of 1966, as amended.